FIG. 7.

Bovine P58^IPK or ΔTPR6 is produced in tumors and tumor cell lines. Immunoblot analysis of P58^IPK and ΔTPR6 protein production was performed. (Left) Four tumors from mice injected with ΔTPR6 cell lines (two from mice injected with ΔTPR6-1-expressing cells, designated ΔTPR6-1.1 and ΔTPR6-1.2, and two from mice injected with ΔTPR6-2-expressing cells, designated ΔTPR6-2.1 and ΔTPR6-2.2) and one tumor from a mouse injected with the P58^IPK-1 cell line (P58^IPK-1) were analyzed by using the anti-P58^IPK monoclonal antibody, 2F8 (5). (Right) Cell lines derived from ΔTPR6-expressing and P58^IPK-overexpressing tumors were analyzed by immunoblotting using the P58^IPK polyclonal antibody. In each panel, cell extracts prepared from MDBK cells were used as a source of bovine P58^IPK protein to serve as a positive control (lane 1). Cell extracts prepared from the NEO cell line were analyzed in parallel as a negative control (lane 2).