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. 2021 Aug 26;12:741839. doi: 10.3389/fimmu.2021.741839

Figure 5.

Figure 5

TRIM65 signaling prevents LPS-induced systemic inflammation and MSU-induced peritonitis and gouty arthritis via suppression of the NLRP3 inflammasome. (A) Wild-type or Trim65-/- mice (5 mice per group) were intraperitoneally injected with LPS (15 mg/kg) and treated for 4.5 h. The sera were collected, and the levels of IL-1β were measured by ELISA. (B, C) Wild-type or Trim65-/- mice (4-5 mice per group) were intraperitoneally injected with MSU crystals (1 mg/mouse) and treated for 6h. The numbers of neutrophils in the peritoneal cavity were analyzed by FACS (B), and the levels of IL-1β (C) were assayed by ELISA. (D–F) Wild-type or Trim65-/- mice (7-10 mice per group) were intraperitoneally injected with MSU crystals, after which joint swelling (D) at different time points was measured with a Vernier caliper, the levels of IL-1β (E) in the supernatants of joint cultures at 24 h were assayed by ELISA, and changes in joint swelling at 24 h were compared (F). Data are shown as the means ± SEM of two or three independent experiments. Student’s t-test, ***P < 0.001.