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. 2021 Aug 26;12:703593. doi: 10.3389/fphys.2021.703593

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Copyright © 2021 Li, Wang, Han, Wu, Zhu, Fang, Bi, Chen, Yao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nell-1 distribution in DPSCs (A–D), HUVECs (E–H), and normal pulp tissues (I–R). (A,E) Normal cell feature of DPSCs and HUVECs. (B,F) DAPI: nucleus stain. (C,G) green fluorescence can be observed in the nucleus of DPSCs and HUVECs. (D,H) Merged images. (I,N) HE staining displayed the morphology and structure of normal rat pulp tissues. (K,L,P,Q) Nell-1, VEGF and Flk-1 can be found in odontoblasts, pulp fibroblasts, and endothelial cells of the blood vessels. (J,O) DAPI: nucleus stain. (M,R) Merging Nell-1 with angiogenetic markers. d, dentin; p, dental pulp; bd, blood vessel.