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. 2021 Aug 26;12:703593. doi: 10.3389/fphys.2021.703593

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Copyright © 2021 Li, Wang, Han, Wu, Zhu, Fang, Bi, Chen, Yao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histology and immunohistochemistry staining. (A) Normal teeth group. (B) Nell-1 group. (C) Collagen group. HE staining showed the cavities, inflamed tissue, and normal structure of rat pulp tissues. The staining of CD31 (D–I) and CD34 (J–L) revealed the blood vessels in pulp tissues. Nell-1 induction group had higher number of blood vessels around cavities than the collagen group, and both of them had fewer blood vessels than negative control group (D–F,J–L). There was no significant difference in the area away from the cavities between three groups (G–I). d, dentin; p, dental pulp; bd, blood vessel.