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Effect of Apaf-1 mutations on the XLucApaf-1 (NLucApaf-1 co-expressed with CLuc Apaf-1) the split luciferase complementation assay. WT or mutant Apaf-1 were expressed in HEK293T cells and the resultant luciferase activity (A) and caspase-3-like activity (B) assessed. The results are expressed as fold change relative to the activity of the WT Apaf-1 and are the mean ± SD of n > 6 experiments.
