Skip to main content
. 2021 Aug 24;6(35):22551–22558. doi: 10.1021/acsomega.1c02274

Figure 2.

Figure 2

Effect of the K192E mutation on Apaf-1: Apaf-1 interactions and caspase activity. WT or mutant Apaf-1 were expressed in HEK293T cells, and the resultant luciferase activity (A) and caspase-3-like activity (B) was assessed. The results are expressed as fold change relative to the activity of the WT Apaf-1 and are the mean ± SD of n > 6 experiments. Luciferase activity of WT Apaf-1 was 32-fold ± 24 greater than the pcDNA3 transfection control (N = 8; P = 0.0087), and caspase activity was 14-fold ± 8.5 greater (N = 6; P = 0.005). (C) Expression levels of the mutants was assessed by immunoblotting for the cotransfected NLuc and Cluc Apaf-1 variants in the HEK293T cells (n > 6, mean ± SD).