Figure 1.

Viability of extracellular and intracellular bacteria and real-time MOI changes in co-culture of E. faecalis and RAW264.7 cells. RAW264.7 cells seeded overnight in 12-well culture plates at 3×10⁴/well were infected with the E. faecalis CA1, CA2, or OG1RF strains at a multiplicity of infection (MOI) of 100:1. Extracellular and intracellular bacteria and RAW264.7 cells were counted at 2, 6, and 12 h of infection. (A) Viability of intracellular (I-strains) and extracellular (E-strains) bacteria [lg (CFU/10⁴ cells)]. (B) Viability of RAW264.7 cells. (C) Real-time MOI of co-culture of E. faecalis and RAW264.7 cells. *P < 0.05, compared to the control group. ^# P < 0.05, compared to the OG1RF group, ^φ P < 0.05 compared to the CA1 group.