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. 2021 Aug 26;11:720147. doi: 10.3389/fcimb.2021.720147

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Copyright © 2021 Chi, Lin, Meng, Tan, Gong and Tong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blots showing the relative expression of PANoptosis-related proteins in RAW264.7 cells 6 and 12 h after E. faecalis infection. (A) Representative immunoblotting bands of eight proteins. Quantification of cleaved caspase-1 (B), cleaved caspase-3 (C), GSDMD-N (D), and pMLKL (E). Relative protein levels were quantified by analyzing the scanned protein bands using the ImageJ software after normalization to β-actin levels on the same blots. All experiments were repeated three times. *P < 0.05 compared to the control group. ^# P < 0.05 compared to the other experimental groups.