Table 3.
Ki-67 Index Values (%) Calculated by Different Methodsa
| Tumor No. | Manual Counting | Digital Image Analysis | |||||
|---|---|---|---|---|---|---|---|
| Observer 1 (DP) | Observer 1 (Ob 1) | Observer 2 (Ob 2) | Observer 3 (DP) | QuantCenter (DP) | HALO (DP) | HALO (Ob 2) | |
| 1 | 6.31 | 6.20 | 5.85 | 5.92 | 5.70 | 4.70 | 5.72 |
| 2 | 1.95 | 2.00 | 2.40 | 1.72 | 3.34 | 2.49 | 3.40 |
| 3 | 1.85 | 2.00 | 1.24 | 1.04 | 2.16 | 2.06 | 1.85 |
| 4 | 24.67 | 23.00 | 23.84 | 22.61 | 24.33 | 20.90 | 36.50 |
| 5 | 0.60 | 1.20 | 1.24 | 0.60 | 0.52 | 0.43 | 1.04 |
| 6 | 1.37 | 1.60 | 1.64 | 1.26 | 2.75 | 1.13 | 1.69 |
| 7 | 3.81 | 4.00 | 4.74 | 3.79 | 3.46 | 3.14 | 4.23 |
| 8 | 4.80 | 5.50 | 4.39 | 5.38 | 5.48 | 4.80 | 3.78 |
| 9 | 6.42 | 9.00 | 5.77 | 7.06 | 6.62 | 2.11 | 5.08 |
| 10 | 2.12 | 3.00 | 2.44 | 2.12 | 1.95 | 1.32 | 1.88 |
| 11 | 8.13 | 9.00 | 4.27 | 7.95 | 4.36 | 3.34 | 3.42 |
| 12 | 5.95 | 8.50 | 7.57 | 6.61 | 6.07 | 4.80 | 6.09 |
| 13 | 0.44 | 0.70 | 0.98 | 0.44 | 1.16 | 0.90 | 1.04 |
| 14 | 11.73 | 10.70 | 9.51 | 11.40 | 10.29 | 3.82 | 7.91 |
| 15 | 8.08 | 10.10 | 8.58 | 7.71 | 6.05 | 4.61 | 8.01 |
| 16 | 2.41 | 4.50 | 2.13 | 2.34 | 2.63 | 2.36 | 1.49 |
| 17 | 3.61 | 2.30 | 4.65 | 4.11 | 4.24 | 4.13 | 4.04 |
| 18 | 1.72 | 1.90 | 2.51 | 1.71 | 1.61 | 1.64 | 1.91 |
| 19 | 3.41 | 2.99 | 4.11 | 3.13 | 1.94 | 1.51 | 2.82 |
| 20 | 2.09 | 2.00 | 2.00 | 1.96 | 1.60 | 1.38 | 1.56 |
| 21 | 14.9 | 15 | 20.2 | — | 23.15 | — | — |
| 22 | 7.9 | 7.1 | 9.8 | — | 13.48 | — | — |
| 23 | 7.1 | 8 | 8.1 | — | 6.45 | — | — |
| 24 | 26 | 16 | 29.6 | — | 27.56 | — | — |
| 25 | 21.1 | 36 | 26.5 | — | 24.5 | — | — |
DP, digital image analysis pathologist; Ob, observer; —, data unavailable.
aThe column names list the method of counting (manual counting vs Quantcenter vs HALO) and the observer performing the count. Listed in parentheses are observers choosing the hotspots. For cases 21 to 25, hotspots were chosen by observer 2.