FIG. 3.

NF-κB-dependent gene expression is stimulated by IL-1 and inhibited by LY294,002. Where indicated, HepG2 cells were incubated with 20 μM LY294,002 for 30 min prior to stimulation with IL-1β for 4 h. (A) Cells were lysed and analyzed for the expression of IL-8 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNAs by the Northern procedure. (B) HepG2 cells were transiently transfected with the NF-κB-dependent reporter plasmid pElam[−143]-luc and analyzed 48 h later. Where indicated, the cells were incubated with 20 μM LY294,002 (LY) for 30 min prior to stimulation with IL-1β for 4 h. Luciferase activity was normalized for transfection efficiency. Data are expressed as fold induction, the ratio between the expression level in the experimental and the level in the untreated control (Con) cells.