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IL-1 stimulates the formation of a p65-p50 heterodimer and a second p65/RelA complex in HepG2 cells. Cells were either left untreated or stimulated with IL-1β for 30 min. The cells were lysed, and the supershift analysis was performed with the antibodies indicated. p65/? is either a homodimer of p65/RelA or a heterodimer of p65/RelA with another NF-κB family member.
