FIG. 9.

Dominant negative Akt blocks IL-1 signaling to an NFκB-dependent promoter. HepG2 cells were transiently cotransfected with the NFκB-dependent reporter plasmid pElam[−143]-luc, together with either a vector or a construct expressing a dominant negative (DN) derivative of Akt. The cells were analyzed 48 h later, with a 4-h stimulation with IL-1β where indicated. Luciferase activity was normalized for transfection efficiency. The data are expressed as fold induction, the ratio of the expression level in the experimental cells and the level in the vector-transfected untreated control cells.