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. 2021 Aug 26;12:705307. doi: 10.3389/fimmu.2021.705307

Figure 7.

Figure 7

BBimfl/fl B cells are resistant apoptosis costimulated with IL-21 and via TLR4, TLR7, TLR9 or CD40. Purified B cells from WT and BBimfl/fl mice were incubated for 48 hours with the indicated TLR agonists or anti-CD40 in the absence or in the presence of IL-21 (25ng/ml) and then analyzed for (A) dead cells and (B) live cells distinguished using a fixable viability dye (Invitrogen). (C) Numbers of live B cells in the cultures (trypan blue-) (D) Splenocytes from BBimfl/fl and WT mice were treated with TLR7 agonist (CL097) or anti-CD40 Abs in the absence or in the presence of IL-21 (25ng/ml), stained with annexin V and 7AAD and analyzed by FCM. Bar graphs showing percentages of B cells that are live (white; 7AAD- Annexin V-) and dead (gray; 7AAD+/- Annexin V+) after culture for 48 hours. Not significant (N.S) P > 0.05, *P ≤ 0.05, calculated by Students T-test.