Table 1.
Antibodies used in this study.
| Ab | Conjugate | Clone | Manufacturer | Catalog No. | Dilution |
|---|---|---|---|---|---|
| B cell antibodies | |||||
| AA4 | PE-Cy7 | AA4.1 | Biolegend | 136506 | 100x |
| B220 | V500 | RA3-6B2 | BD Bioscience | 561226 | 200x |
| CD19 | PE-CF594 | ID3 | BD Bioscience | 562291 | 200x |
| CD21 | PE | 7g6 | BD Bioscience | 552957 | 200x |
| CD23 | PerCP-CY5.5 | 3B4 | Biolegend | 101618 | 200x |
| CD138 | APC | 281-2 | Biolegend | 142506 | 100x |
| IgD | Pac Blue | 11-26c.2a | Biolegend | 405711 | 400x |
| IgM | BV711 | ll/41 | BD Bioscience | 743327 | 400x |
| T cell antibodies | |||||
| CD3 | BV605 | ucht1 | Biolegend | 300459 | 100x |
| CD4 | BV711 | GK1.5 | BD Bioscience | 563050 | 100x |
| CD8 | BV605 | 53-6.7 | BD Bioscience | 563152 | 100x |
| CD44 | PE | im7 | eBioscience | 12-0441-83 | 200x |
| CD62L | Alexa Fluor 700 | mel-14 | eBioscience | 56-0621-82 | 200x |
| Cytokine, proliferation and viability antibodies | |||||
| Annexin V | FITC | – | Biolegend | 640922 | – |
| 7AAD | – | – | Biolegend | 640922 | – |
| IFNα | FITC | RMMA-1 | PBL assay science | 22100-3 | 100x |
| IFNβ | FITC | MMHB-3 | PBL assay science | 21400-3 | 100x |
| IFNγ | BV786 | Q31-378 | BD Bioscience | 564723 | 100x |
| IL-6 | PE | MP5-20F3 | MP5-20F3 | 504504 | 100x |
| L-10 | BV605 | JES5-16E3 | BD Bioscience | 564082 | 100x |
| Ki-67 | PE-Cy7 | B56 | BD Bioscience | 561283 | 100x |
| Immunohistochemistry Antibodies | |||||
| Goat anti-mouse CD3 | – | M20 | Santa Cruz Biotechnology | SC-1127 | 100x |
| CD19 | Biotin | ID3 | BD Bioscience | 553784 | 100x |
| Anti-goat | Alexa Fluor 647 | – | Life Technologies | A-21447 | 1000x |
| Streptavidin | Alexa Fluor 488 | – | Biolegend | 405235 | 100x |