Fig. 2.

Conserved RRKK motif mediates TMIGD1 binding with ERM proteins moesin and ezrin. A Amino acid sequence homology of human and mouse TMIGD1 and sequence of the known moesin binding receptors. B Schematic of wild type GST-TMIGD1 and GST-RRKK motif mutant TMIGD1 (4A-TMIGD1). C Equal amount of purified recombinant Glutathione S-transferase (GST), GST-TMIGD1 and 4A-TMIGD1 fusion proteins were resolved on SDS-PAGE followed by Coommassie blue staining. D Whole cell lysates (WCL) derived from HEK-293 cells expressing empty vector (EV), GFP-tagged moesin (MSN-GFP) or GFP-tagged ezrin were incubated with GST, GST-TMIGD1 or GST-4A-TMIGD1 followed by western blot analysis using an anti-GFP antibody. The same membranes were blotted for GST or GAPDH