Fig. 5.

TMIGD1 stimulates α-tubulin acetylation and regulates mitotic spindle assembly. A EV/786-0 and TMIGD1/786-0 cells were stained with an antibody to K40 acetyl-α-tubulin (green) and phalloidin/actin (red). B Cell lysates from the same cell groups were blotted for acetylated-α-tubulin, total α-tubulin and TMIGD1. The graph represents three independent experiments ***p < 0.001. C EV/786-0 and TMIGD1/786-0 cells (triplicate per group) were treated with nocodazole (0.5 µg/ml) for various times, followed by staining with an antibody against acetylated-α-tubulin. The numbers of cells displaying intact microtubules were manually counted (n = 50 cells per each group of triplicates). D EV/786-0 and TMIGD1/786-0 cells were stained with acetylated-α-tubulin (green) and phalloidin/actin (red) and the pictures of mitotic cells (n = 20 cells per group) were taken under an epifluorescence microscope