Figure 4.

Immunohistochemical staining for lipogenesis marker PPAR-α (A, B, and C) and apoptotic marker caspase-3 (E, F, and G) in liver tissues in negative, NASH, and ADF groups. Magnification, ×400. Scale bar = 50 µm. Arrows: immunopositive cells. Gel photographs of the studied genes PPAR-α (D) and caspase-3 (H). PPAR-α qPCR product (141 bp), caspase-3 (110 bp), and GAPDH (85 bp). Image of GAPDH gel electrophoresis was repeated with images of other genes as it was used as a control gene. Abbreviations: L, ladder; NASH, non-alcoholic steatohepatitis; ADF, alternate-day fasting; PPAR-α, peroxisome proliferator-activated receptor-α; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.