Figure 1.

Experimental design of the study. Workflow used in the study to enrich for CTCs and CNV profiling using Ampli1 WGA and LowPass kit for Illumina. Following enrichment (EpCAM-dependent vs size-based platforms), single NEN CTCs and matched WBC are selectively recovered in dynamically controlled dielectrophoretic cages using the DEPArray Image-Assisted Digital Cell Sorter. CTC samples undergo WGA and QC prior to low-resolution whole-genome sequencing for CNV profiling. Where surgical resection or biopsy specimens are available, samples are processed for bulk LPWGS and single-cell LPWGS as per CTCs.