Fig. 1.
Dynamic aspects of tau inclusion formation. Doxycycline-inducible GFP-0N4R (a) and 4RD-YFP (b) tau reporter cell lines express human tau with aggregation prone mutations P301L and P301L/V377M, respectively. The cells were seeded with brain fractions including seed-competent pathogenic tau derived from TgTauP301L mice with a CSA Type 2 denaturation profile for sarkosyl-insoluble tau. Droplet-like movement of intracellular tau inclusions (a) and transfer through a tunneling nanotube-like membrane extension (b) were analyzed by time-lapse imaging of the seeded cells for 5 h (5 min/frame for 60 frames). Z4 and Z7 are different focal planes with the 3-μm interval. Scale bars, 10 μm