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. 2021 Sep 9;19:199. doi: 10.1186/s12915-021-01132-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Disruption of nuclear-cytoplasmic compartmentalization in ES1 cells. a Schematic illustration of nuclear-cytoplasmic compartmentalization (NCC) reporter construct (top). 4RD-YFP tau reporter (Ctrl) and ES1 cells transiently transfected with the NCC reporter (bottom). Control cells exhibited segregated fluorescent signals, green cytoplasm and red nuclei (colored in magenta), whereas red fluorescence signals were colocalized with cytoplasmic green fluorescence in ES1 cells, indicating a defect in NCC. CV, the coefficient of variation of the mean pixel intensity of red fluorescence; RFP, red fluorescent protein; GFP, green fluorescent protein. Scale bar, 10 μm. b, c The mean pixel intensity of RFP (b) and the CV (c) in the transfected reporter (n = 71) and ES1 cells (n = 48). Cells from 8 different areas, respectively (160 μm² for each, see Supplementary Figure 7). a.u., arbitrary unit. Scale bar, 10 μm