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. 2021 Sep 4;15:3735–3747. doi: 10.2147/DDDT.S314767

Figure 2.

Figure 2

BM prevented apoptosis in rat chondrocytes treated with 25 mM TBHP. (A and B) Chondrocytes were stained with JC-1 and measured by flow cytometry to determine mitochondrial membrane potential. The results showed that 0.025 and 0.05 µM BM effectively protected mitochondrial damage in rat chondrocytes treated with 25 mM THBP. (E) Represent images of chondrocytes stained with JC-1 under confocal microscopy. Scale bar, 50 µm. Red fluorescence represented normal membrane potential, and green fluorescence represented mitochondrial membrane potential depolarization. (C and D) Chondrocytes were stained with Annexin V/PI and measured by flow cytometry to detect cell apoptosis. The results showed that 0.025 and 0.05 µM BM significantly inhibited apoptosis of rat chondrocytes treated with 25 mM THBP. *P < 0.05 and **P < 0.01.