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Immunohistochemical analysis of the cartilage explants cultured ex vivo. (A) Representative images of collagen II and aggrecan immunochemistry at magnification of 10× (scale bar, 1mm) and 100× (scale bar, 50 µm). (B) 0.025 and 0.05 µM BM significantly increased collagen II and aggrecan positive cell ratio, suggesting BM alleviated TBHP-induced ECM degradation in cartilage explants cultured ex vivo. *P < 0.05, **P < 0.01 and ****P < 0.001.
