Fig. 4.

Confirm FOXA1 binds to the miR-4721 promoter region. A Schematic diagram of the miR-4721 promoter region with putative FOXA1 TFBS (A, B, and C) and wild-type (WT) and TFBS mutants (MutAB, MutAC, MutBC, and MutABC). B CHIP experiment shows 3 binding sites of FOXA1 in miR-4721 promoter region. C Amplification of miR-4721-binding sites after Ch-IP using an antibody against FOXA1. An IgG antibody was used as the negative control. D EMSA assay of FOXA1 binding to miR-4721 promoter in NPC cells. E The expression level of miR-4721 in NPC cells overexpressing FOXA1 was detected by qPCR. F Relative luciferase activity of the indicated promoter vectors HONE1 and SUNE1 cells transfected with FOXA1 plasmids. *P < 0.05