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. 2021 Sep 9;19:200. doi: 10.1186/s12915-021-01118-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Data quality control for analysis of targeted metabolomics and the distribution of the metabolite concentrations in all the FFs. A The distribution of the cumulative frequency of the CV for the relative retention time in the QC samples. The Y-axis represents the cumulative frequency of the CV, and the X-axis denotes the CVs for the relative retention time. B The distribution of the cumulative frequency of the CV for the metabolite concentrations in the QC samples. The Y-axis represents the cumulative frequency, and the X-axis stands for the CV for the metabolite concentrations. C, D Concentration boxplots for all the targeted metabolites quantified in all the FFs. The Y-axis represents the concentrations (ng/ml) after log10 transformation, and the X-axis denotes the metabolites. AA, amino acid; ACR, acyl carnitine; BA, biological amine; HO, hormone; SP, sphingolipid; HE, hexose; Vit, water-soluble and fat-soluble vitamins; PC, phosphatidylcholine