Table 2.

The effect of LEAP-2 on GH release based on experiments.

Treatment	GH Detection method	Effect on GH secretion	Model	Ref.
i.p. LEAP-2 (doses range between 0.72~360 nmol/kg), 10 min later, i.v. 6 nmol/kg ghrelin	At 0-, 5-, 10-, 15-, 30-, and 60-min post ghrelin injection	Inhibited ghrelin-induced GH secretion from a dose higher than 7.2 nmol/kg	3–4 months old male C57BL/6 mice	(5)
LEAP-2 overexpression by AAV	1-point GH detection under the condition of a 40% chronic caloric restriction	Both GH and ghrelin levels were lower than control
i.p. 10 mg/kg BW LEAP-2 monoclonal antibodies following 24h fasting	Sampling at an interval of 20 min for 2h	Increased total and peak GH
i.v. 15 nmol LEAP-2, 10 min later, i.v. 5 nmol ghrelin	At 0, 15-, and 30-min post ghrelin injection	Inhibited ghrelin-induced GH secretion	8 weeks old male Wistar rats	(19)
1 µM LEAP-2 +/- 0.1 µM ghrelin add to primary pituitary cells	1 point GH detection	Inhibited ghrelin-induced GH secretion

i.p, intraperitoneal injection; i.v, intravenous injection; AAV, Adeno-associated virus.