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. 1999 Jul;19(7):4832–4842. doi: 10.1128/mcb.19.7.4832

FIG. 3.

FIG. 3

DSBs in inserts at different locations on chromosome III detected by using pBR322 sequences as probes. (A) DSBs between URA3 and arg4 sequences (DSB-left). DNA was digested with PstI (P), which cuts in pBR322 and in URA3, and probed with a PstI-AlwNI fragment from pBR322. DSB-left is indicated by a solid arrow, and the ARG4 promoter (prom.) is indicated by a dotted arrow. Mitotic DNA is from MJL2105. Meiotic DNAs are, from left to right: MJL2105, MJL2144, MJL2237, MJL1185, MJL2139, MJL2106, MJL1176, MJL1170, MJL2324, and MJL2326. (B) DSBs in the pBR322 sequence downstream of the arg4 fragment (DSB-right). DNA was cut with StuI (St) and a locus-specific enzyme: RSV161, SacII; YCR004c, AflII; YCR017c, AflII; CHA1, XhoI; YCR026c, AflII; RIM1, SphI; MAT, XbaI; YCL011c, AflII; LEU2, AflII; and HIS4, Bpu1102I. The probe used was a HindIII-BamHI pBR322 fragment. DSB-right is indicated by solid arrows and the ARG4 promoter is indicated by a dotted arrow. Mitotic DNA is from MJL2105. Meiotic DNAs are, from left to right: MJL2105, MJL2144, MJL2237, MJL2139, MJL1185, MJL1176, MJL2324, MJL2326, and MJL1170.