TABLE 2.
URA3-arg4 plasmids
| Plasmid namesa | Locus | Fragment coordinatesb | Integration sitec |
|---|---|---|---|
| pMJ462 and pMJ349 | YCL011c | 101706–103145 | 102553 (AflII) |
| pMJ508 and pMJ510 | YCR004c | 118532–119248 | 118810 (AflII) |
| pMJ481 and pMJ484 | RVS161 | 129959–130492 | 130199 (SacII) |
| pMJ514 and pMJ520 | YCR017c | 144280–144630 | 144409 (AflII) |
| pMJ482 and pMJ485 | YCR026c | 163445–164151 | 163812 (AflII) |
| pMJ483 and pMJ486 | RIM1 | 171197–172008 | 171587 (SphI) |
a
Plasmids are derived from pMJ113 (arg4-nsp) and pMJ115 (arg4-bgl), respectively (67).
b
Coordinates on chromosome III (51) of the locus-specific fragment used to direct integration. In all cases, this fragment was inserted at the pBR322 EcoRI site (see Materials and Methods).
c
Chromosome III coordinate of integration site and restriction enzyme used to direct integration.