Figure 1.

SHR2554 inhibited proliferation, induced G1 phase arrest and promoted apoptosis in DLBCL cell lines. (a) Eleven DLBCL cell lines were treated with indicated concentrations of SHR2554 for 6 days. Then the cell viability was measured by Cell Titer-Glo luminescent cell viability assay. Viable cells were calculated by dosing/vehicle × 100%. (b,c) Cells were treated with indicated concentrations of SHR2554 for 2 days. Then cell cycle was assessed by flow cytometry and cell-cycle-related proteins were detected by Western blot. (d,e) Cells were treated with indicated concentrations of SHR2554 for 6 days. Then apoptosis cells were assessed by flow cytometry and apoptosis-related proteins were detected by Western blot. Data are expressed as mean ± SD of three independent experiments and representative figures are presented. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with vehicle group. Detailed information about Western Blot can be found at supplementary materials.