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. 2021 Aug 24;13(17):4265. doi: 10.3390/cancers13174265

Figure 2.

Figure 2

Generation of recombinant CRAd with MUC16 1040 bp upstream fragment to control E1A expression. (A). Illustration of CRAd genome recombination. E1A promoter of Ad5 was replaced by the MUC16 1040 fragment. HSV-TK was inserted in the E3 region. (B). Ad5/MUC16-1040/TK-EGFP was packaged using HEK293 cells and assessed for viral plague formation and EGFP expression, scale bar: 200 μm, magnification: 40×. (C). Oncolysis (CPE) of HeLa cells, scale bar: 200 μm, magnification: 40×. (D). Oncolysis (CPE) of OVCAR3 cells, scale bar: 50 μm, magnification: 200×. (E). GCV-induced cell death in the virus-infected mouse ovarian cancer line ID8 cells, *** p = 0.0007 vs. Ctrl, n = 3.