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. 2021 Aug 24;13(17):4265. doi: 10.3390/cancers13174265

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Replication of Ad5/MUC16-1040/TK-EGFP is dependent on CA-125 expression in a panel of selected ovarian cancer cell lines. (A). Relative MUC16 mRNA expression in selected cell lines. (B). Oncolysis induced by Ad5/MUC16-1040/TK-EGFP in selected human ovarian cancer cell lines. Coomassie blue stain assay: Live cells attached to plate culture surface were stained blue. Dead cells floated and were washed out, leaving the culture surface blank and devoid of stain. Intensity of blue indicates cell density. Punctuated white areas in the SKOV3 cells were non-specific detachment of cells. MOI: multiplicity of infection, virus:cell infection ratio. (C). E1A expression in selected ovarian cancer cell lines infected with Ad5/MUC16-1040/TK-EGFP.