Figure 1.
BAP1 binds active gene promoters and is excluded from Polycomb repressive domains
(A) Heatmaps representing ChIP-seq intensity of the indicated proteins in wild-type ESCs.
(B) Venn diagram of HA-BAP1, SUZ12, and RING1B target genes in ESCs.
(C) Genome-wide functional annotation of peaks generated from the indicated ChIP-seq analyses.
(D) Boxplots showing the expression levels obtained from RNA-seq analyses in WT mESCs for the clusters of target genes generated in (A).
(E) Genome browser snapshot of ChIP-seq tracks showing an example of mutual exclusivity of PRC1/2 and PR-DUB target genes.
(F) Western blot analysis with the indicated antibodies on total protein extracts from the indicated rescue ESC cell lines (E14 WT + empty vector, Bap1 KO + empty vector, Bap1 KO + BAP1 WT, Bap1 KO + BAP1 C91S).
(G) Volcano plots of −log10 (p value) against log2 fold change representing the differences in gene expression in the indicated cell lines.
(H) Percentage overlap of differentially expressed genes (DEGs) from (G) with either HA-BAP1, RING1B, or SUZ12 ChIP-seq targets.