Figure 3.

PCGF3/5-PRC1 complexes are the major source of BAP1-opposed H2AK119ub1 deposition

(A) Western blot analysis with the indicated antibodies on total protein extracts from the indicated ESC lines.

(B) Metaplots and heatmaps representing normalized ChIP-seq intensity for H2AK119ub1.

(C) Boxplot of normalized intensity profiles for H2AK119ub1 ChIP-seq in the indicated cell lines.

(D) Genome browser snapshot of H2AK119ub1 ChIP-seq in the indicated cell lines.

(E) Boxplots representing H2AK119ub1 ChIP-seq RPKM levels in the indicated cell lines at intergenic sites (n = 38,068).

(F) Boxplots showing the log2 fold change RPKM ratio for H2AK119ub1 in the indicated cell line comparisons at intergenic regions (n = 38,068).