FIG 1.

Diagram of the HPV18 replicon and the HPV18 URR cis element insertion. (A) Diagram of the HPV18 replicon plasmid, pCGneo. Prokaryotic elements are highlighted in orange (R6K ori, I-EC2K, and CER), while eukaryotic elements are highlighted in violet (simian virus 40 [SV40] promoter-enhancer) and green (beta-globin polyA signal). The neomycin resistance gene is in blue. The HPV18 central URR and origin are represented in black, with E1 and E2 binding sites indicated by red and yellow bars, respectively. The BstXI restriction enzyme sites used for Southern blot analysis are indicated. (B) Diagram of the HPV18 central URR and 3′ URR origin sequences inserted into the replicon. The four regions of the central URR are indicated, and nucleotide numbers are from the HPV18 genome. The arrow represents the early viral P105 promoter. (C) A global alignment of the central URR and ori from the nine Alphapapillomavirus 7 species of human papillomaviruses using the Geneious Prime multiple sequence alignment algorithm of the central and 3′-terminal half of the URR. The HPV18 central URR and origin elements are indicated. Nucleotides within the sequences are color-coded as indicated. A mean pairwise identify score (sliding window size of 1) for each nucleotide is shown graphically above the aligned sequences and indicate less than 30% (red), 30% to 99% (yellow), or 100% (green) nucleotide conservation among the sequences.