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. 2021 Sep 9;95(19):e01012-21. doi: 10.1128/JVI.01012-21

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FIG 5 — Advanced A26 complexes with A27 and excludes F13 incorporation. Cells were infected with the indicated viruses for the indicated length of time, and lysates were analyzed by SDS-PAGE under denaturing (A) and nondenaturing (B) conditions for the expression of the viral proteins F13 (V5), A26 (FLAG), and A27 as well as the early protein C6, used as a control. Molecular weights (kDa) are indicated on the left. In agreement with their promoter configuration, A26 expression was enhanced in vA26^pF13 viruses, whereas F13 expression was slightly delayed in vF13^pA26 viruses. Where A26 expression was advanced, it correlated with increased detection of A26-A27 complexes. (C) Cells were infected with WT-analog (WT) and vA26^pF13 viruses for the indicated length of time, and progeny virus was purified and analyzed by SDS-PAGE under denaturing and nondenaturing conditions for the expression of the indicated proteins. Progeny virus from vA26^pF13 infection incorporated significantly higher levels of A26 that correlated with a decrease in F13 detection.