FIG 3.

CD4-CTL response requires late viral replication. ECTV-infected mice were treated with PBS or 600 μg of cidofovir intraperitoneally at 3 dpi. (A and B) Virus titers in the liver (A) and spleen (B) at 8 dpi. (C and D) Percentage of CD44⁺ in CD4 (C) or CD8 (D) T cells in the liver and spleen at 8 dpi. (E and F) Frequency of GzmB⁺ in CD4 T cells (E) and CD8 T cells (F) in the liver and spleen at day 8 after infection. Unpaired t tests were conducted on all graphs, and asterisks (*) denote statistical differences. Compiled data are displayed as means ± the SEM representing three independent experiments (n = 12 to 15).