FIG 7.

CD4-CTLs upregulate Runx3 and maintain ThPOK expression. (A) Splenocytes from naive mice were stained with antibodies targeting the indicated transcription factors. Histogram plots are shown for both CD4 and CD8 T cells compared to isotype antibody staining. (B) Relative transcription numbers, represented as fragments per kilobase of transcripts per million map reads (FPKM), for indicated genes for T cell populations analyzed as in Fig. 6. The data are presented as means ± the SEM from the three RNA samples collected. (C) Histograms of antibody staining for transcription factors for indicated splenic T cell populations from infected mice at 7 dpi. (D) Splenic CD4-CTLs at 7 dpi were first gated on Runx3-positive cells, followed by ThPOK gating. Isotype antibody staining on CD4 T cells was used to set gates. Concatenated or representative flow plots gated on three to four mice were used for histograms, and the results are representative of two independent experiments.