FIG. 1.

Characterization of the MMC sensitivities of FA-A and FA-G lymphoblast lines. The full-length FANCG cDNA (7, 25) was isolated by RT-PCR of RNA from a human MG63 tumor cell line and subcloned into the murine retroviral vector pMMP(puro) (19). The indicated retroviral supernatants were generated and used to infect FA lymphoblast lines, and puromycin-resistant cells were selected. The cells analyzed included EUFA316 (FA-G) cells, HSC72 (FA-A) cells, and normal (PD7) cells. Consistent with previous studies (7), pMMP-FANCG infection also resulted in functional complementation (correction of MMC sensitivity) of another FA-G cell line, EUFA143 (data not shown). WT, wild type.