FIG. 2.

Expression of FANCG in FA-G lymphoblasts restores the FANCA/FANCC interaction. Whole-cell extracts (WCE) were generated from lymphoblast lines, including EUFA316, EUFA316-FANCG, EUFA143, EUFA143-FANCG, and PD7 (normal adult control). These protein extracts (100 μg) were probed directly by immunoblotting with either anti-FANCA or anti-FANCC serum. The FANCA protein is indicated by an arrow, and additional bands in the anti-FANCA immunoblot are nonspecific. Alternatively, the same amount of protein from each extract (2 mg) was used for immunoprecipitation with affinity-purified anti-FANCC serum as indicated. Immune complexes were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and immunoblotted with anti-FANCA or anti-FANCC serum. WT, wild type; IP, immunoprecipitation. The values to the right of the gel are molecular sizes in kilodaltons.