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Binding of FANCA and FANCG in vitro. In vitro-translated, [35S]methionine-labeled FANCA (A), FANCG (G), and FANCC (C) were prepared in separate reactions and mixed as indicated (Input Protein). Immunoprecipitation was done with either preimmune (P), anti-FANCA (A), anti-FANCG (G), or anti-FANCC (C) serum. Total reticulocyte lysate (without immunoprecipitation) containing labeled FANCA, FANCG, and FANCC was loaded in lanes 13, 14, and 15, respectively. IP, immunoprecipitating.
