FIG. 6.

Analysis of the FANCA/FANCG/FANCC protein complex in cell lines from multiple FA complementation groups. (A) Whole-cell extracts were prepared from the indicated lymphoblast lines, and proteins were immunoprecipitated with either preimmune (P) or anti-FANCA (A) serum. Immune complexes were immunoblotted with antiserum to FANCA, FANCG, or FANCC, as indicated. The cell lines analyzed included normal control PD7 cells (lanes 1 and 2) and HSC72 (lanes 3 and 4), HSC72–FA-A (lanes 5 and 6), BD32 (lanes 7 and 8), HSC230 (lanes 9 and 10), PD4 (lanes 11 and 12), HSC536 (lanes 13 and 14), PD20L (lanes 15 and 16), EUFA130 (lanes 17 and 18), EUFA121 (lanes 19 and 20), EUFA316 (lanes 21 and 22), and EUFA173 (lanes 23 and 24) cells. The BD32 (FA-A) cell line expressed a mutant FANCA polypeptide [FANCA(H1110P)]. The HSC536 (FA-C) cell line expressed a mutant FANCC polypeptide [FANCC(L554P)]. All of the cell lines examined expressed comparatively equal levels of FANCC protein as judged by whole-cell lysate immunoblotting (data not shown), except PD4 cells, which expressed no FANCC protein. IP, immunoprecipitating; IgG, immunoglobulin G. WT, wild type.