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Nuclear localization of the FANCA and FANCG polypeptides. The human fibroblast line GM0637, derived from a normal (wild-type) control, was infected with either pMMP-FANCA (wild type) (A) or pMMP-FANCG (wild type) (B) as indicated. Pools of infected cells were stained with anti-FANCA or anti-FANCG serum or stained with the DNA-specific dye 4′,6-diamidino-2-phenylindole (DAPI) and analyzed by immunofluorescence assay as previously described (29).
