Fig. 2.

Whole spleen RNAseq of RMT and SBP-infected mice in the first week of infection reveals subtle differences.

A. Principal component analysis (PCA) of gene expression from the top 1000 variable genes between the transmission types and the timepoints. PC1 and PC2 are used and depict the greatest variation in gene expression between the timepoints and transmissions. Colours indicate the transmission type: pink (SBP), blue (RMT) and black (naïve), whilst symbols indicate days in the blood (0, 1, 2, 3, 4, and 6). Three distinct clusters can be seen with samples clustering by days post-infection. B. Venn diagram showing differential gene overlaps of all protein-coding genes between the transmission routes. Differentially expressed genes (DEGs) were identified by comparing the transmission routes against the naïve control (log₂ fold change > 1 or < −1, FDR < 0.05). C. Venn Diagrams showing differences in DEGs identified in RMT and SBP on specific days post-infection.