Fig. 3.

Increased activation of genes associated with interferon pathways and myeloid cell activation in spleens of mice infected with RMT P. chabaudi parasites.

A. Innate DEG Heatmap. Gene expression signature of DEGs identified by comparing infection timepoints against controls. DEGs were then compared to the InnateDB database. Shown are samples from day 4 (n = 3), day 6 (n = 4) from RMT and SBP initiated infections, and naïve samples (n = 4). DEGs compared against naïve samples were selected for the heatmap. Colours represent the Z-score values, which is a measurement of the number of standard deviations a sample value is above or below the mean across all samples for a given gene. B. Number of transcripts per million (TPM) of a selection of genes of interest encoding cytokines/chemokines, interferon-related genes, or genes involved in macrophage activation and found as immune-associated genes with the InnateDB in RMT spleens (blue bars) or SBP spleens (pink bars). Lines above groups with asterisks illustrate significances between groups. *, p < 0.05; **, p < 0.01; ***, p < 0.001.