Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 26;8:709741. doi: 10.3389/fcvm.2021.709741

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Xiao, Zha, Zhang, Wu, Chen, Yang, Yang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

GLP-1 postpones the age-induced mineralization of AVICs. To determine the effect of GLP-1 on age-induced mineralization in AVICs, primary AVICs were cultured for 7, 14, and 21 days in osteogenic medium plus GLP-1 (100 pmol/L) or not. The group without the GLP-1 treatment was used as a control (Con). (A) Alizarin Red S stained the calcification cells, and the pictures were taken using Image-Pro Plus 6.0. Alizarin Red S staining is shown at 400 × magnification. (B) Quantification of Alizarin Red S staining. (C) Quantification of ALP activation in AVICs. The dye was extracted and quantified as described in the Methods section (n = 6, mean ± SD, **P < 0.01 compared with Con; ^#P < 0.05, ^##P < 0.01 compared with 7-day culture; ^@P < 0.05, ^@@P < 0.01 compared with 14-day culture).