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. 2021 Aug 26;8:709741. doi: 10.3389/fcvm.2021.709741

Figure 5.

Figure 5

GLP-1 regulates the expression of calcification-related genes. The primary AVICs were stimulated with or without GLP-1 (100 pmol/L) for 21 days in osteogenic medium. The group without the GLP-1 treatment was used as a control (Con). (A) RUNX2, MSX2, SOX9, BMP2, and BMP4 were detected by real-time PCR. (B) The protein expression and location of RUNX2, MSX2, SOX9, BMP2, and BMP4 were determined by immunofluorescence analysis. RUNX2, MSX2, and SOX9 were assessed using Alexa Fluor 488-conjugated secondary antibody (Green); BMP2 and BMP4 were assessed using Alexa Fluor 549-conjugated secondary antibody (Red). (C) RUNX2, MSX2, SOX9, BMP2, and BMP4 were detected by western blot. The results are representative of three independent experiments. Real-time PCR (A) and western blot (C) quantified as relative units (genes or proteins/β-actin) (n = 3, mean ± SD, **P < 0.01 compared with Con).