Table 1. Cell surface associated component genes’ expression in 630Δerm 72-hour biofilm cells, compared to planktonically grown cells.
Gene ID | Gene name | Gene function/product | Fold change |
---|---|---|---|
Fatty acid biogenesis | |||
CD1183 | acpP | ACP | 9.92 |
CD1062 | acpP | ACP | 9.45 |
Cell wall | |||
CD2766 | lcpB | Ligase anchoring polysaccharide to the cell wall | 24.93 |
CD2762 | uppS | Putative undecaprynyl phosphate synthetase | −1.23 |
Exported proteins | |||
CD2831 | Putative adhesin | 6.77 | |
CD2830 | zmp1 | Extracellular zinc metalloprotease | −1.16 |
CD0873 | Adhesin and sugar binding lipoprotein (ABC transport) | 65.34 | |
Type IV pili biogenesis | |||
CD3513 | pilA1 | Putative pilin protein | 6.11 |
CD2305 | pilW | Putative pilin protein | 11.31 |
CD3504 | Putative type IV prepilin peptidase, A24A family | 6.45 | |
CD3506 | Conserved hypothetical protein | 6.19 | |
CD3507 | Putative type IV pilin | 10.78 | |
CD3508 | Putative type IV pilin | 12.91 | |
CD3509 | Putative type IV pilus assembly protein | 10.13 | |
CD3510 | Putative membrane protein | 9.78 | |
CD3511 | Putative type IV pilus secretion protein | 9.25 | |
Flagellum biogenesis | |||
CD0234 | csrA | Carbon storage regulator homolog CsrA | −1.35 |
CD0254 | flgD | Basal-body rod modification protein FlgD | −1.24 |
CD0255 | flgE | Flagellar hook protein FlbE (distal rod protein) | −1.29 |
CD0255A | flbD | Flagellar protein FlbD | −1.28 |
CD0263 | flhA | Flagellar biosynthesis protein FlhA | −1.19 |
630Δerm biofilm was grown in a continuous flow microfermentor for 72 hours with gene expression measured by microarray. Negative integers represent decreased expression. (Data from [52] transformed to show absolute fold changes).
ACP, acyl carrier protein.