Extended Data Fig. 3. T_reg purification.

T_reg-cells were isolated from the mesenteric lymph nodes of Foxp3^Cre and Tcf7^fl/flFoxp3^Cre mice. (a) Schematic representation of magnetic purification of T_reg-cells, and FACS analysis showing over 90% purity. (b) Expression changes of the Tcf7 transcripts between TCF-1-deficient and TCF-1-sufficient T_reg-cells. The color intensity is proportional to the average gene expression across cells in the indicated T_reg cluster. The size of circles is proportional to percentage of cells expressing indicated genes.