Extended Data Fig. 5. TCF-1-deficient and sufficient T_reg-cells show distinct effector functions.

T_reg-cells were isolated from the mesenteric lymph nodes of Foxp3^Cre and Tcf7^fl/flFoxp3^Cre mice. (a) mRNA expression of Maf projected on the UMAP, comparing T_reg-cells derived from Foxp3^Cre to Tcf7^fl/fl Foxp3^Cre mice. (b) Violin plots showing expression of Maf in individual T_reg clusters. (c) GSEA of MAF downregulated genes and T_H17 pathway defined by Stubbington. (d) Kegg IL17 signaling pathway projected on UMAP, comparing TCF-1-sufficient and TCF-1-deficient T_reg-cells (e) GSEA analysis for the Kegg IL17 signaling pathway comparing transcriptomes of TCF-1-sufficient and TCF-1-deficient T_reg-cells across all cell types. Normalized enrichment scores (NES) are color coded. −log₁₀ (FDR) values are proportional to the circle size. FDR>15% are masked with gray color. (fgh) mRNA expression of Ccr9, Erdr1 and Igfbp4 projected on the UMAP, comparing TCF-1-sufficient and TCF-1-deficient Klf2⁻ cells for the Kegg IL17 pathway.