Extended Data Fig 1. TCF-1 deficiency selectively reprograms T_reg-cells without compromising their core signature.

T_reg-cells were isolated from the mesenteric lymph nodes of Foxp3^Cre and Tcf7^fl/flFoxp3^Cre mice. (a) Representative FACS histograms of MLN purified cells from Foxp3^Cre Tcf7^fl/fl and control Foxp3^Cre showing selective loss of TCF-1 from T_reg-cells in Foxp3^Cre Tcf7^fl/fl mice. (b and c) Histogram plots showing the cumulative data of the same. (b: n = 4; p < 0.0001 & c: n = 5) Data are representative of two independent experiments and n represents biologically independent replicate mice; means ± SEM; two-sided, unpaired t-test. (d) GSEA plot comparing the enrichment of genes expressed more strongly in Foxp3^Cre versus Foxp3^CreTcf7^fl/fl T_reg-cells.