FIGURE 6.

Silica and LPS affect TAC in a differential manner. RAW 264.7 macrophages were treated with LPS (10 ng/ml) or silica (50 μg/cm²) or left untreated for 6 h. (A–L) Intracellular relative amount of TAC (A–F) metabolites and (G–J) amino acids. (K and L) Changes in succinate and glutamate atomic percent enrichment at steady state determined by LC-HRMS. Atomic percent enrichment was calculated using the MIMOSA method. Data are the mean ± SEM of six samples; n = 6; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, control versus LPS versus silica.